Anagramme & Informationen zu | Englisch Wort AMPLICONS

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Beispiele für die Verwendung von AMPLICONS in einem Satz

• Analysis of amplicons has been made possible by the development of amplification methods such as PCR, and increasingly by cheaper and more high-throughput technologies for DNA sequencing or next-generation sequencing, such as ion semiconductor sequencing, popularly referred to as the brand of the developer, Ion Torrent.
• Electrophoretic separation of amplicons on a gel matrix, followed by visualisation of the band pattern.
• If a and b are the signals from two amplicons in the patient sample, and A and B are the corresponding amplicons in the experimental control, then the dosage quotient DQ = (a/b) / (A/B).
• Alternatively, if amplicon sizes overlap, the different amplicons may be differentiated and visualised using primers that have been dyed with different colour fluorescent dyes.
• Denaturing gradient gel electrophoresis (DGGE) is a microbial fingerprinting technique that separates amplicons of roughly the same size based on sequence properties When the fragment reaches its melting point (threshold of enough denaturant), it stops moving.
• To further amplify the cDNA for RNA-seq, the cDNA amplicons generated by the original PCR step undergoes in vitro transcription using the T7 promoter incorporated in the ad1 primer to ensure RNA transcripts come from cDNA, not gDNA.

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